摘要：目的 研究移植骨髓间充质干细胞(MSCs)对肺气肿大鼠血浆肿瘤坏死因子-α(TNF-α)水平的影响.方法 将30只Wistar大鼠随机分为对照组、肺气肿组、肺气肿+MSCs治疗组,每组30只.在气管内注入脂多糖(LPS)并烟雾暴露,建立肺气肿大鼠模型,然后将培养的MSCs经尾静脉注入到肺气肿大鼠体内.观察移植后大鼠肺组织的变化,酶联免疫吸附测定(ELISA)法检测血浆TNF-α含量.结果 肺气肿组、肺气肿+MSCs移植组均出现肺气肿样病理改变,但后者较前者明显减轻.与肺气肿组比较,肺气肿+MSCs组TNF-α含量显著降低.结论 MSCs移植对肺气肿大鼠有保护作用. Abstract： Objective To investigate the effect of bone marrow mesenchymal stem cell (MSCs) transplantation on plasma TNF-α levels in rats of pulmonary emphysema. Methods Thirty wistar rats were randomized into three groupes:normal control group, pulmonary emphysema group, pulmonary emphysema +MSCs transplantation groupe. The pulmonary emphysema model of rats were established by intratracheal instillation of lipopolysaccharide twice and daily exposure to smog. Bone marrow MSCs were infused through tail vein. Morphologic changes of the lung tissues were observed. The level of plasma TNF-α were dectected using enzyme-linked immunosorbent assay. Results Destruction of alveolar walls was observed in rat lungs from pulmonary emphysema group and pulmonary emphysema +MSCs transplantation group. MSCs transplantation group significantly ameliorated the emphysematous changes. Significant differences in the levels of TNF-α between pulmonary emphysema group and pulmonary emphysema +MSCs transplantation group were observed. Conclusions MSCs therapy shows protective effects against pulmonary emphysema.

关键词：骨髓间充质干细胞移植 肺气肿大鼠模型 大鼠血浆 stem cell 

单位：郑州大学第一附属医院呼吸科 450052 河南省胸科医院