摘要：目的 探讨瘦素(Lep)和神经肽Y(NPY)在子痫前期患者胎盘组织中的表达变化及意义.方法 应用免疫组织化学染色及图像分析方法检测子痫前期40例(子痫前期组:其中轻度子痫前期18例,重度子痫前期22例)和正常妊娠妇女20例(对照组)胎盘组织中Lep和NPY的表达及定量.结果 ①子痫前期组胎盘组织Lep表达灰度(46±3)较对照组(27±3)高,差异有统计学意义(t＝21.143,P＜0.01);NPY表达灰度(44±4)较对照组(48±4)低,差异亦有统计学意义(t＝3.431,P＜0.01).②轻、重度子痫前期组之间相比较,Lep和NPY的差异均无统计学意义(P＝0.253,P＝0.396).③Lep和NPY表达水平呈负相关(r＝-0.477,P＜0.01).结论 Lep通过自分泌和(或)旁分泌作用抑制胎盘组织NPY的表达,参与了子痫前期的发病. Abstract： Objective To investigate the expression of leptin(Lep) and neuropeptide Y(NPY) in pre-eclamptic placenta and its significance. Methods The expression of Lep and NPY in placenta of 40 mild or sever pre-elampsia cases and 20 normal pregnancy cases were detected by immunohistochemisty staining and image analysis. Results ①Intensity of Lep immnostaining in trophoblast was significantly higher in pre-eclampsia group(46±3) than that in normal controls(27±3) (P＜0.01), but NPY was significantly lower in pre-eclampsia group(44±4) whan that in normal controls(48±4)(P＜0.01). ②There was no significant difference in immunostaining for either Lep or NPY between the mild and severe pre-eclampsia group(P＞0.05). ③NPY level showed negative correlation with Lep(r＝-0.477,P＜0.01). Conclusions Lep may downregulate NPY in placenta by autocrine and(or) paracrine, and consequently influence pathogenesis of pre-eclampsia.

关键词：子痫前期患者 胎盘组织 瘦素 神经肽 npy表达 

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